The tissues are embedded in paraffin, cut into 3–6 micron sections, placed on glass microscopic slides and stained. The standard stain in dermatopathology is. Methods. A total of histopathological specimens collected from 66 combat-injured United States military personnel with IFIs were. Pathol Res Pract. May; Histological technique in routine histopathology. An opinion. Henriques U. "Routine technique" means fixation in.


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Histological technique in routine histopathology. An opinion.

The sections are then stained, and examined with the light microscope. Square tissue blocks about 1cm in each dimensionor whole organs, are fixed by chemical fixation: The chemical added binds to and cross-links some proteins, and denatures other proteins through dehydration.

Histopathological techniques hardens the tissue, and inactivates enzymes that might otherwise degrade the tissue. Fixation also kills bacteria etc. It can also enhance tissue staining. To cut sections, the tissue has to histopathological techniques embedded in paraffin wax, but wax is not soluble in water or alcohol.

However, it is soluble in a paraffin solvent called 'xylene'.


Hematoxylin, a basic histopathological techniques, stains nuclei blue due to an affinity to nucleic acids in the cell nucleus; eosin, an acidic dye, stains the cytoplasm histopathological techniques. Uranyl acetate and lead citrate are commonly used to impart contrast to tissue in the electron microscope.

There are many other staining techniques that have been used to selectively stain cells and cellular components. One of these techniques involves marking peripheral tumors or surgical margins, in which a certain color of dye is applied to the posterior border of a sample, another to the anterior, etc.

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Other compounds used to color tissue sections include safraninOil Red OCongo redFast green FCF histopathological techniques, silver salts, and numerous natural and artificial dyes that usually originated from the development of dyes for the textile industry. Histochemistry refers to the science of using chemical reactions histopathological techniques laboratory chemicals and components within tissue.

Histological Techniques

A commonly performed histochemical technique is the Perls Prussian blue reaction, used histopathological techniques demonstrate iron deposits in diseases like hemochromatosis.

Histology histopathological techniques have often been examined by radioactive techniques. In historadiographya slide sometimes stained histochemically is X-rayed.

More commonly, histopathological techniques is used to visualize the locations to which a radioactive substance has been transported within the body, such as cells in S phase undergoing DNA replication which incorporate tritiated thymidineor sites to which radiolabeled nucleic acid probes bind in in situ hybridization.

For autoradiography on a microscopic level, the slide is typically dipped into liquid nuclear tract emulsion, which dries to form the exposure film.

Individual silver histopathological techniques in the film are visualized with dark field microscopy. Recently, antibodies have been used to specifically visualize proteins, carbohydrates, and lipids.

Histological technique in routine histopathology. An opinion.

This process is called immunohistochemistryor when the stain is a fluorescent molecule, immunofluorescence. Sections are cut with histopathological techniques or diamond knives and mounted on copper mesh grids.

Histopathological techniques some cases, serial sections are required. For this technique, multiple consecutive sections are prepared as slides. Using serial sections allows the 3D structure of the tissue to be visualized. This is especially important in determining whether an abnormality is an artifact of preparation or a pathologic process.

A wide variety of procedure are included within this frame, some resulting in excellent preparations, others, although sometimes presented in scientific publications, giving slides which are virtually useless for the histopathological techniques of differential diagnostic evaluation.

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